RESUMEN
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Asunto(s)
Humanos , Masculino , Femenino , Niño , Fragaria/efectos adversos , Hipersensibilidad a los Alimentos/diagnóstico , Hipersensibilidad a los Alimentos/etiología , Hipersensibilidad Inmediata , Región MediterráneaAsunto(s)
Botulismo/epidemiología , Brotes de Enfermedades/estadística & datos numéricos , Animales , Aspartato Aminotransferasas/sangre , Austria/epidemiología , Biomarcadores , Toxinas Botulínicas/sangre , Botulismo/diagnóstico , Botulismo/transmisión , Clostridium botulinum/aislamiento & purificación , Criopreservación , Heces/microbiología , Manipulación de Alimentos , Microbiología de Alimentos , Conservación de Alimentos , Fragaria/efectos adversos , Fragaria/microbiología , Humanos , Carne/efectos adversos , Carne/microbiología , Productos de la Carne/efectos adversos , Productos de la Carne/microbiología , Ratones , Ratones Endogámicos BALB C , Sus scrofaRESUMEN
BACKGROUND: No strawberry allergen has so far been identified and characterized. METHODS: Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh and frozen strawberries were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation. RESULTS: Several IgE-binding proteins could be detected. In more than half of the patient sera, a 20/18-kDa doublet band was observed in Western blotting. These two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20/18-kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell-based assay of patient blood samples, basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1. CONCLUSIONS: We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.
